/ /

  • linkedin
  • Increase Font
  • Sharebar

    Protein fluorescence explored for cataract diagnosis


    To explore this proposition, Duncan and colleagues examined the fluorescence of PTMs in lenses from pigs and in donated human lenses.

    They found that due to the spectral selectivity on the red edge of the Trp absorption spectrum, they could excite a fraction of Trp residues situated in hydrophilic pockets with a red-shifted absorption spectrum. A 317 nm light-emitting diode (LED) was effective for this excitation. They also measured fluorescence from Trp photo-degradation products and ArgP.Courtesy of Dr Rory Duncan

    They compared pig lenses irradiated with ultraviolet light to untreated lenses. And they compared human lenses from an eye bank, post-operational phaco-emulsified lens samples and clear emulsified lenses of 40 and 46 year old patients who underwent cosmetic surgery.

    “The technology identifies how much oxidative damage lens proteins have accumulated through lifestyle or environmental factors,” said Duncan in the press release. “This accumulated damage may be important in determining risk factors for a number of age-related conditions.”

    In summarizing, they wrote that they had developed a novel non-invasive method of semi-quantitative determination of the concentrations of fluorescent PTMs in the lens based on simultaneous measurements of fluorescence emission.

    “We show that the fluorescence spectra of the individual fluorescent components normalised against Trp intensity enable determination of their relative concentration with an accuracy of 5–10%,” they wrote. “This makes this method useful for cataract grading and for monitoring cataractogenesis over a period of time.”

    By monitoring changes in the lens structure at the molecular level, the hoped to also facilitate the development of medications that might slow down the development of cataracts.


    New Call-to-action


    You must be signed in to leave a comment. Registering is fast and free!

    All comments must follow the ModernMedicine Network community rules and terms of use, and will be moderated. ModernMedicine reserves the right to use the comments we receive, in whole or in part,in any medium. See also the Terms of Use, Privacy Policy and Community FAQ.

    • No comments available


    View Results